Custom DNA
Manufacturing

High-Fidelity Amplification of Customer-Provided DNA Constructs

PlasmidFactory offers specialized services in the production of plasmid and minicircle DNA, with a core focus on the precise amplification and purification of DNA constructs supplied by our clients. As a dedicated manufacturer we work with fully defined sequences or ready-to-use plasmids to ensure consistent, high-quality yields.

While we do not provide de novo plasmid design, our platform is optimized to accommodate a wide range of DNA sequences, including those with structurally complex or regulatory-sensitive elements. Our robust processes maintain the integrity of constructs containing:

• Inverted terminal repeats (ITRs)
• Polyadenylation signals (polyA)
• Repeat-rich or GC-rich regions
• Non-coding regulatory sequences

In addition, we offer targeted modifications within defined parameters. These include:

• Substitution of the antibiotic resistance cassette
• Exchange of small, non-coding vector components
• Adaptation of selection markers, if compatible with the existing vector architecture

If your project requires initial gene synthesis, we can facilitate this step as well. Once the plasmid is finalized, PlasmidFactory ensures scalable, reproducible DNA production at Research Grade, CCC Grade, High Quality Grade, or GMP-compliant levels - with full traceability and quality control.

Our manufacturing process utilizes biotechnological amplification in Escherichia coli to ensure:

• High-fidelity replication using natural proofreading mechanisms
• Minimized risks of sequence errors or point mutations
• Avoidance of synthetic enzymes or ligases
• Efficient scale-up without additional purification burdens
• Proprietary, highly efficient downstream processing

This results in highly pure, topologically correct DNA with a predominance of the supercoiled form – ideal for transfection efficiency and clinical-grade applications.

All custom DNA undergoes stringent in-house quality controls, with the option to include additional, project-specific tests (non exhaustive list):

• DNA purity
• DNA topology: supercoiled vs open circular
• Endotoxin levels
• DNA yield and concentration
• NGS sequencing
• Host cell protein (via ELISA)
• Residual chromosomal DNA (via qPCR)
• RNA contamination analysis
• Many more

All tests are documented in a batch-specific report.