A powerful, fast and reliable analytical separation technique to evaluate plasmid topologies within your plasmid DNA sample
Capillary Gel Electrophoresis (CGE) is an advanced analytical technique designed to separate and characterize biomolecules based on their size and conformation. Especially in the field of plasmid DNA analysis, CGE offers unmatched precision in resolving different plasmid forms. At its core, CGE utilizes narrow capillaries filled with a gel-like polymer matrix and applies a high-voltage electric field to enable rapid, high-resolution separation of plasmid species. Compared to conventional gel electrophoresis, CGE is faster, more sensitive, and highly reproducible - making it a preferred method in quality control and structural characterization of plasmid DNA.
At PlasmidFactory, we have taken CGE a significant step further. Our proprietary CGE method has been refined over many years of experience and is tailored specifically for the analysis of plasmid DNA, setting a new benchmark for precision and consistency. Unlike generic CGE systems, our technology is finely optimized for plasmid topological assessment and allows us to distinguish and quantify subtle conformational differences with extraordinary accuracy. This makes our CGE Service one of the most reliable and advanced solutions currently available for detailed plasmid quality evaluation.
Capillary gel electropherogram and different types of plasmid topology:
A – B: ccc-forms (monomer and dimer)C – D: linear-forms (monomer and dimer)E - F: oc-forms (monomer and dimer)
We offer CGE analysis as a standalone service or in combination with our comprehensive contract manufacturing packages, allowing full flexibility based on your needs. Whether you're producing your own plasmid DNA or working with our custom production services, our CGE analysis can provide you with the insights you need to move forward with your project.
Our proprietary CGE platform allows for the accurate identification and quantification of the three primary plasmid forms:
- ccc-monomer (covalently closed circular monomer)
- ccc-dimer (covalently closed circular dimer)
- oc-forms (open circular)
These topological variants are critical quality attributes, particularly for therapeutic applications, gene therapy, and high-end research, where the presence of unwanted forms can compromise function, transfection efficiency, or regulatory compliance.
The CGE Service is an integral part of our standard quality control process for our CCC grade plasmids as well as for all HQ plasmid products. For clients working with Research grade plasmids, the CGE analysis can be added upon request. This modular approach allows you to match analytical rigor with project requirements and budget.
Capilary Gel Electrophoresis as Integral Part of your Stability Studies
CGE service is available as part of our Storage & Logistics Service, where it is used to monitor the long-term stability of plasmid DNA samples. This is especially valuable when plasmid batches are stored over extended periods or transported under varied environmental conditions. Periodic CGE checks ensure that your plasmid preparations remain within defined quality thresholds, adding an extra layer of security to your project planning and thus supporting your stability studies. Ask for our publication on plasmid stability!
In summary, CGE is not just another quality control step - it is a core analytical tool that helps you verify, validate, and optimize your plasmid DNA. With PlasmidFactory’s proprietary CGE technology, you gain access to one of the most advanced analytical services for plasmid topology currently available.
Take advantage of our long-standing expertise and discover how our CGE Service can enhance your research, production, and regulatory processes. Contact us today to learn how our solutions can be tailored to your specific application.
Get in touch!
Insights
CGE analysis is a proprietary analytical tool of PlasmidFactory and is the only reliable method to quantify plasmid forms.
Scientific research is one of the most exciting and rewarding of occupations. It is like a voyage of discovery into unknown lands, seeking not for new territory but for new knowledge. It should appeal to those with a good sense of adventure.
Let us know how
we can help.
Get in touch so we can assist you in a targeted manner — either by filling out this online form or emailing us at:
contact@PlasmidFactory.com
I would like personalised advice on the following topic …
- Manufacturing of customized plasmid DNA
- Manufacturing of customized minicircle DNA
- In-Stock products for AAV production
- In-Stock products (reporter-plasmids/-minicircle)
- cooperation opportunities
- Other topic
I would like a specific quote for the following product
- Customized DNA
- AAV plasmids und minicircles
- reporter gene plasmids and minicircles
- pEPito plasmids
- Molecular Size Markers
- CGE Service
- Storage Service
Select
dialogue
provide required
information
receive
offer
We are aware of our responsibility towards our fellow human beings and the environment. We act in accordance with general ESG (Environmental Social Governance) criteria.
