Custom DNA:
Our manufacturing service for your custom DNA, tailored to your requirements – available in Research grade, CCC grade, High Quality (HQ) grade, and GMP grade.
- Plasmid DNA
  Production of your plasmid DNA with our optimized manufacturing workflows and customized solutions after pre-order consultation.
- Minicircle DNA
  A minimal, supercoiled monomeric circular expression cassette with your gene of interest, free of bacterial backbone, providing demonstrated advantages over conventional plasmids.
In-Stock DNA:
Preproduced plasmid and minicircle DNA available off-the-shelf for immediate delivery.
- Plasmids & Minicircles for AAV production
  Plasmids containing both helper and packaging functions as well as vector plasmids and minicircles for the production of various AAV serotypes.
- Reporter constructs and pEPito plasmids
  Plasmids and minicircles with different reporter genes for transfection optimization as well as pEPito plasmids with S/MAR elements to stabilize gene expression.
- Molecular Size Markers
  Lyophilized DNA ladders with different size ranges for the analysis of short and long fragments –loading buffer included.
Analysis & Storage:
Analysis and storage services to complement our DNA manufacturing service – also available for external DNA.
- CGE Service
  Capillary gel electrophoresis-based quantification of the topology of your plasmid DNA.
- Storage Service
  GMP-compliant storage of your DNA samples in our facility.

DNA Storage
Service

Your plasmid DNA is stored according to ICH guidelines in our GMP storage facility.

Quote available on request:

Included

Transfer of plasmid DNA to a continuously monitored and validated depot at a constant temperature ≤ -20°C
All parameters in accordance with ICH guidelines
Permanent and GMP-compliant control and documentation

Additional

Stability is monitored by AGE (Agarose Gel Electrophoresis) and CGE (Capillary Gel Electrophoresis)
Storage at different temperatures
Just-in-time delivery on dry ice

We safeguard your DNA during long-term storage under GMP-compliant conditions.

Get in touch!

Other Products
& Services

Plasmid DNA

Minicircle DNA

Plasmids & Minicircles for AAV production

Reporter constructs and pEPito plasmids

Molecular Size Markers

Analysis & Storage

CGE Service

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Manufacturing of customized plasmid DNA
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In-Stock products for AAV production
In-Stock products (reporter-plasmids/-minicircle)
cooperation opportunities

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Customized DNA
AAV plasmids und minicircles
reporter gene plasmids and minicircles
pEPito plasmids
Molecular Size Markers
CGE Service
Storage Service

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Useful background information

The most important terms relating to minicircle DNA and plasmids explained in detail. Useful background information for smooth communication.

We are aware of our responsibility towards our fellow human beings and the environment. We act in accordance with general ESG (Environmental Social Governance) criteria.

Publications about Minicircle DNA

The following literature on the topics plasmids, DNA manufacturing, gene therapy, and genetic vaccination can be requested at PlasmidFactory (contact@plasmidfactory.com). Books can only be purchased through the bookstore.

M. Izco, M. Schleef, M. Schmeer, E. Carlos, G. Verona and L. Alvarez-Erviti, Targeted Extracellular Vesicle Gene Therapy for Modulating Alpha-Synuclein Expression in Gut and Spinal Cord, Pharmaceutics 2023, 15, 1230 (2023)

R. Shankar, N. Schäffer, C. Krüsemann, M. Schmeer, M. Schleef, In-process controls for plasmid isoform characterization, Abstract ESGCT 29th Annual Congress in collaboration with BSGCT Edinburgh, UK October 11–14, 2022 Published Online:14 Dec 2022

D. Lock , R. Monjezi, C. Brandes , S. Bates, S. Lennartz, K. Teppert , L. Gehrke, R. Karasakalidou-Seidt, T. Lukic, M. Schmeer, M. Schleef, N. Werchau, M. Eyrich , M. Assenmacher, A. Kaiser, S. Prommersberger, T. Schaser, M. Hudecek, Automated, scaled, transposon-based production of CAR T cells, open access, Journal for Immuno Therapy of Cancer (2022)

S. Prommersberger, R. Monjezi, R. Shankar, M. Schmeer, M. Hudecek, Z. Ivics et al., Minicircles for CAR T Cell Production by Sleeping Beauty Transposition: A Technological Overview, Gene Therapy Of Cancer – Methods and Protocols (2022)

M. Schmeer, M. Schleef, R. Shankar, D. Kobelt, W. Walther, Capillary Gel Electrophoresis (CGE) for Quality Control of Plasmid DNA in Gene Therapy: Quality Control of 20 Years Stored GMP-Grade Plasmid DNA, Gene Therapy Of Cancer – Methods and Protocols (2022)

R. Shankar, N. Schäffer, M. Schmeer, J.M. Risse, K. Friehs & M. Schleef, Recombinant expression of Barnase in Escherichia coli and its application in plasmid purification. Microb Cell Fact 20, 171 (2021).

M. Izco, J. Blesa, M. Schleef, M. Schmeer et al., Systemic Exosomal Delivery of shRNA Minicircles Prevents Parkinsonian Pathology, Molecular Therapy Vol.27 No 12 Dec., The American Society of Gene and Cell Therapy (2019)

T. Buchholz, M. Schleef (2018), Classification and Regulation of Advanced Therapy Medicinal Products, International Biopharmaceutical, Industry, Volume 1 Issue 3, 44-45, Winter 2018

T. Buchholz, C.J. Christel and H. Besir (2018), Customised Viral Engineering - Proactive Progress, European Biopharmaceutical Review, April, 24-31.

M. Holstein, C. Mesa-Nuñez, C. Miskey et al. (2018), Efficient Non-viral Gene Delivery into Human Hematopoietic Stem Cells by Minicircle Sleeping Beauty Transposon Vectors, Molecular Therapy Vol. 26 No 4, 1137-1153, April 2018

T. Buchholz, M. Schmeer and M. Schleef (2017), Size Matters, European Biopharmaceutical Review, October, 68-70.

M. Schmeer, T. Buchholz and M. Schleef (2017), Plasmid DNA Manufacturing for Indirect and Direct Clinical Applications, Human Gene Therapy, 28(10):856-861.

R. Shankar, M. Schmeer and M. Schleef (2017), Minicircles: next-generation gene vectors, Cell Gene Therapy Insights 017;3(2), 285-300.

Wiebke Garrels, Thirumala R. Talluri, Maren Ziegler, Ilka Most, Diego O. Forcato, Marco Schmeer, Martin Schleef, Zoltán Ivics and Wilfried A. Kues (2016), Cytoplasmic injection of murine zygotes with Sleeping Beauty transposon plasmids and minicircles results in the efficient generation of germline transgenic mice, www.biotechnology-journal.com

M. Schnödt, M. Schmeer, B. Kracher, C. Krüsemann, L. E. Espinosa, A. Grünert, T. Fuchsluger, A. Rischmüller, M. Schleef and H. Büning (2016), DNA Minicircle Technology Improves Purity of Adeno-associated Viral Vector Preparations, Molecular Therapy—Nucleic Acids, 5, e355.

R. Monjezi, C. Miskey, T. Gogishvili, M. Schleef, M. Schmeer, H. Einsele, Z. Ivics and M. Hudecek (2016), Enhanced CAR T-cell engineering using non-viral Sleeping Beauty transposition from minicircle vectors, Leukemia, 1-9.

I. Freudenau, P. Lutter, R. Baier, M.Schleef, H. Bednarz, A. R. Lara and K. Niehaus (2015), ColE1-plasmid production in Escherichia coli: mathematical simulation and experimental validation, Frontiers in Bioengineering and Biotechnology, 10.3389/fbioe.2015.00127.

H. Akita, D. Kurihara, M.Schmeer, M. Schleef and H. Harashima (2015), Effect of the Compaction and the Size of DNA on the Nuclear Transfer Efficiency after Microinjection in Synchronized Cells, Pharmaceutics, 10.3390/pharmaceutics7020064.

M. Schmeer, M. Schleef (2014), Pharmaceutical Grade Large-Scale Plasmid DNA Manufacturing Process, in: M. Rinaldi et al. (eds), Methods in Molecular Biology, DNA Vaccines, vol. 1143, 219-240.

D. Kolbelt, M. Schleef, M. Schmeer, J. Aumann, P.M. Schlag,W. Walther (2013), Performance of High Quality Minicircle DNA for In Vitro and In Vivo Gene Transfer, Mol. Biotechnol., 53 (1), 80-89.

S. Chabot, J. Orio, M. Schmeer, M. Schleef, M. Golzio, J. Teissié (2013), Minicircle DNA Electrotransfer for efficient Tissue targeted Gene Delivery, Gene Therapy 20 (1), 62-68.

M. Viefhues, S. Wegener, A. Rischmüller, M. Schleef and D. Anselmetti (2013), Dielectrophoresis based continuous-flow nano sorter: fast quality control of gene vaccines, Lab Chip, 13 3111-3118.

M. Schleef (2013), Non Viral DNA Vectors, In: D. Scherman (ed.), Advanced Textbook on Gene Transfer, Gene Therapy and Genetic Pharmacology – Principles, Delivery and Pharmacology and Biomedical Application of Nucleotide-Based Therapies, Imperial College Press London, 183-205.

M. Schleef (2013), Production and Purification of Plasmid Vectors, In: D. Scherman (ed.), Advanced Textbook on Gene Transfer, Gene Therapy and Genetic Pharmacology – Principles, Delivery and Pharmacology and Biomedical Application of Nucleotide-Based Therapies, Imperial College Press London, 517-525.

W. Walther, M. Schmeer, D. Kobelt, R. Baier, A. Harder, V. Walhorn, D. Anselmetti, J. Aumann, I. Fichtner, M. Schleef, A seven-Year Storage Report of Good Manufacturing Practice-Grade Naked Plasmid DNA: Stability, Topology, and In Vitro/In Vivo Functional Analysis, HGT Clin. Dev. 24, 147-153.

M. Schleef (ed.) (2013), Minicircle and Miniplasmid DNA Vectors, The Future of Non-Viral and Viral Gene Transfer, Wiley-VCH Weinheim, ISBN 978-3-527-32456-9.

M. Schleef, M. Blaesen, M. Schmeer, R. Baier, C. Marie, G. Gickson, D. Scherman (2010), Production of non-viral DNA Vectors, Current Gene Therapy, 10, 487-507. Press London, 183-205.

M. Schmeer (2009), Electroporative Gene Transfer, in: W. Walther and U. S. Stein (eds), Methods in Molecular Biology, Gene Therapy of Cancer, vol. 542, 157-165. Press London, 183-205.

M. Schleef, M. Blaesen (2009), Production of Plasmid DNA as a Pharmaceutical, in: W. Walther and U. S. Stein (eds), Methods in Molecular Biology, Gene Therapy of Cancer, vol. 542, 471-495.

C. Maucksch, A. Bohla, F. Hoffmann, M. Schleef, M. K. Aneja, M. Elfinger, D. Hartl, C. Rudolph (2009), Transgene expression of transfected supercoiled plasmid DNA concatemers in mammalian cells, J Gene Med 11: 444-453.

M. Schleef, R. Baier, W. Walther, M.L. Michel, and M. Schmeer (2006), Long-Term Stability Study and Topology Analysis of Plasmid DNA By Capillary Gel Electrophoresis, BioProcess International, 38-40.

M. Schleef (ed.) (2005), DNA-Pharmaceuticals, Wiley-VCH Weinheim, ISBN 3-527-31187-4.