The pDG plasmids for the production of AAVs form a 2-plasmid transfection system. This means that in contrast to triple transfection with three different plasmids, only two components are needed: A plasmid with the gene of interest to be transferred, flanked by ITRs (vector plasmid) and the corresponding pDG plasmid containing all the information for the propagation and packaging of the gene of interest (the so-called helper & packaging plasmid). Additional infection with helper viruses – required in the original systems – is not necessary as the Adenovirus helper functions are included in the pDG plasmids.