AAV Packaging and Helper Plasmids for highest Packaging Capacities
PlasmidFactory offers plasmid and minicircle DNA tailored specifically for AAV production workflows, including not only AAV transfer plasmids, but also the packaging and pHelper plasmids, the latter two combined on one construct - providing essential components for efficient virus packaging systems. Our plasmids are ideal for customers looking to produce AAV in research or (pre)clinical settings. These DNA elements are optimized for transfection into cells for packaging, enabling reliable AAV transfer and robust expression of transgenes.
Our vector system supports AAV rep and cap expression, AAV genome delivery, and integration of your transgene of choice. With compatibility across different AAV serotypes and capsids, our plasmids can help researchers study tissue tropism, transduction efficiency, and transgene expression in non-dividing cells. AAVs require the precise coordination of AAV replication, capsid protein production, and adenovirus helper functions to mediate AAV replication and viral genome replication without the need for a helper virus like adenovirus.
Every AAV viral vector constructed using our plasmids includes well-defined ITRs, ensuring efficient packaging within the typical capacity. Whether you're exploring different AAV variants, optimizing for tropism, or aiming to transduce specific tissues, our tools support AAV manufacture without compromising the integrity of the host genome.
The plasmids used in AAV workflows are widely adopted to encode genes of interest for therapeutic and basic research purposes. Although we do not supply AAV particles, our materials are designed to streamline and support your AAV manufacturing process with scientific reliability.
PlasmidFactory is the exclusive worldwide provider of this 2-Plasmid-System or production of AAV vectors and holds the exclusive license from the German Cancer Research Centre (DKFZ) for global production, distribution, and use of Helper & Packaging plasmids of the pDG and pDP family.
We offer a large variety of Packaging & Helper plasmids for the production of serotype-specific AAV vectors, also including frequently used AAV5 and AAV6. These constructs are available from our In-Stock service for various capsids.
Advances in the field of gene therapy have demonstrated the potential of viral vectors to treat various monogenic, inherited diseases in human patients. Thanks to their unique characteristics, recombinant vectors derived from adeno-associated viruses (AAVs) represent a particularly effective technology for delivering therapeutic genes to their targets. Optimizing production of these recombinant AAV vectors to meet the demand for increasingly large quantities, while maintaining quality and cost-effectiveness, is a key challenge.
Our In-Stock service currently includes plasmids to produce serotypes 1-6, 8, and 9. We offer complete analysis of your DNA by LPS assay, BCA assay, UV spectroscopy, restriction analysis, and detection of RNA and bacterial chromosomal DNA by agarose gel electrophoresis.
These plasmids are also available in HQ (High Quality) grade for clinical applications.
In-Stock:
Helper & Packaging Plasmids & Minicircles for Production of various AAV Serotypes
Helper & Packaging/Vector constructs
All In-Stock products are pre-produced in Research Grade Quality and High Quality Grade are therefore available at short notice. Different rep and cap combinations are available. Minimum order quantity 5 mg (pDNA) or 0.5 mg (minicircle DNA). GMP Quality Grade on request.
| Item No. | Name | Comment | Vector size |
|---|---|---|---|
Item No. PF0400 | Name pDM | Comment Serotype: rep2/cap2 wild type: rep (rep89, 68, 52, 49) ITR of AAV2, no RFP | Vector size 21.5 kbp |
Item No. PF0401 | Name pDP1rs | Comment Serotype: rep2/cap1 RFP | Vector size 23.7 kbp |
Item No. PF0402 | Name pDP2rs | Comment Serotype: rep2/cap2 RFP | Vector size 23.7 kbp |
Item No. PF0403 | Name pDP3rs | Comment Serotype: rep2/cap3 RFP | Vector size 23.7 kbp |
Item No. PF0404 | Name pDP4rs | Comment Serotype: rep4/cap4 RFP | Vector size 23.1 kbp |
Item No. PF0405 | Name pDP5rs | Comment Serotype: rep2/cap5 RFP | Vector size 23.7 kbp |
Item No. PF0406 | Name pDP6rs | Comment Serotype: rep2/cap6 RFP | Vector size 23.7 kbp |
Item No. PF0431 | Name pDP1 | Comment Serotype: rep2/cap1 like pDP1rs, no RFP | Vector size 21.9 kbp |
Item No. PF0421 | Name pDG (=pDP2) | Comment Serotype: rep2/cap2 like pDP2rs, no RFP | Vector size 21.8 kbp |
Item No. PF0433.2 | Name pDP3.2 | Comment Serotype: rep2/cap3 like pDP3rs, no RFP | Vector size 21.9 kbp |
Item No. PF0434 | Name pDP4 | Comment Serotype: rep4/cap4 like pDP4rs, no RFP | Vector size 21.2 kbp |
Item No. PF0435 | Name pDP5 | Comment Serotype: rep2/cap5 like pDP5rs, no RFP | Vector size 21.8 kbp |
Item No. PF0436 | Name pDP6 | Comment Serotype: rep2/cap6 like pDP6rs, no RFP | Vector size 21.9 kbp |
Item No. PF0478 | Name pDP8.ape | Comment Serotype: rep2/cap8 | Vector size 22.0 kbp |
Item No. PF0439 | Name pDP9 | Comment Serotype: rep2/cap9 | Vector size 21.8 kbp |
Item No. PF0422 | Name pDG (R484E/R585E) | Comment Serotype: rep2/cap2 like pDG (PF0421), but without HSGAG binding motif | Vector size 19.2 kbp |
Item No. PF1451 | Name pAAV-ssGFP | Comment vector plasmid with AAV-ITRs, CMV promotor, GFP, selection marker (Amp or Kan) | Vector size 8.0 kbp |
Item No. PF1500 | Name pAAV-scGFP | Comment vector plasmid with AAV-ITRs, CMV promotor, GFP, selection marker (Amp or Kan) | Vector size 6.0 kbp |
Item No. MC3051 | Name MC.AAV-ssGFP | Comment AAV-ITRs, CMV promotor, GFP, no bacterial backbone (e.g., no resistance genes) | Vector size 4.5 kbp |
Item No. MC3064 | Name MC.AAV-scGFP | Comment AAV-ITRs, CMV promotor, GFP, no bacterial backbone (e.g., no resistance genes) | Vector size 2.5 kbp |
The 2-Plasmid-System for AAV production offers a cost-effective solution, maximizing efficiency and minimizing expenses.
PlasmidFactory has obtained an exclusive, worldwide license for the production, distribution, and use of the Helper & Packaging plasmids of the pDG and pDP families from the German Cancer Research Centre (DKFZ).
AAV vector plasmids – keeping ITRs intact for highest AAV Packaging Capacity
PlasmidFactory’s proprietary ITRRESCUE® technology enables amplification of pDNA containing sensi-tive inverted terminal repeats (ITRs). While restriction digestion with SmaI is the standard quality control for ITR sequences, this method is not 100% accurate and does not allow quantification of the fraction of non-intact plasmids. PlasmidFactory’s self-developed Next Generation Sequencing (NGS) technology complements our fermentation process, allowing sequencing and quantification of ITR sequences while maintaining intact plasmids. GFP reporter gene cassettes with ITR2 flanked single strandet (ss) or self-complementary (sc) reporter genes are available In-Stock as minicircle or plasmid DNA.
(Fig. artistic representation)
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Gene Delivery Vehicles – AAVs for long-term gene expression
Adeno-associated viruses have great potential in the field of gene therapy and are frequently produced in high-titer in HEK293 cells. The use of minicircles improves the quality of viral vector preparations and increases production efficiency.
When Minicircles were used for AAV manufacture we got an increase of the genomic titer between 300-400% and an increase of the yield per cell.
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