Plasmids & Minicircles
for AAV production

Optimisation of AAV production with our exclusive, proprietary 2-Plasmid-System

PlasmidFactory is the exclusive worldwide provider of the 2-Plasmid-System for production of AAV vectors and holds the exclusive license from the German Cancer Research Centre (DKFZ) for global production, distribution, and use of Helper & Packaging plasmids of the pDG and pDP family.

We offer a large variety of Helper & Packaging plasmids for the production of serotype-specific AAV vectors. These plasmids are available from our In-Stock service.

Advances in the field of gene therapy have demonstrated the potential of viral vectors to treat various monogenic, inherited diseases in human patients. Thanks to their unique characteristics, recombinant vectors derived from adeno-associated viruses (AAVs) represent a particularly effective technology for delivering therapeutic genes to their targets. Optimizing production of these recombinant AAV vectors to meet the demand for increasingly large quantities, while maintaining quality and cost-effectiveness, is a key challenge.

Our In-Stock service currently includes Helper & Packaging plasmids to produce serotypes 1-6, 8, and 9. We offer complete characterization of your DNA by LPS assay, BCA assay, UV spectroscopy, restriction analysis, and detection of RNA and bacterial chromosomal DNA by agarose gel electrophoresis.

These plasmids are also available on request in HQ (High Quality) grade for clinical applications.

In-Stock:

Plasmids & Minicircles for AAV production

Helper & Packaging/Vector constructs

All In-Stock products are pre-produced in Research grade quality and are therefore available at short notice. Minimum order quantity 5 mg (plasmid DNA) or 0.5 mg (minicircle DNA). Other quality grades on request.

Get table as PDF
Item No.NameCommentVector size
Item No.
PF0400
Name
pDM
Comment
Serotype: rep2/cap2 wild type: rep (rep89, 68, 52, 49) ITR of AAV2, no RFP
Vector size
21.5 kbp
Item No.
PF0401
Name
pDP1rs
Comment
Serotype: rep2/cap1 RFP
Vector size
23.7 kbp
Item No.
PF0402
Name
pDP2rs
Comment
Serotype: rep2/cap2 RFP
Vector size
23.7 kbp
Item No.
PF0403
Name
pDP3rs
Comment
Serotype: rep2/cap3 RFP
Vector size
23.7 kbp
Item No.
PF0404
Name
pDP4rs
Comment
Serotype: rep4/cap4 RFP
Vector size
23.1 kbp
Item No.
PF0405
Name
pDP5rs
Comment
Serotype: rep2/cap5 RFP
Vector size
23.7 kbp
Item No.
PF0406
Name
pDP6rs
Comment
Serotype: rep2/cap6 RFP
Vector size
23.7 kbp
Item No.
PF0431
Name
pDP1
Comment
Serotype: rep2/cap1 like pDP1rs, no RFP
Vector size
21.9 kbp
Item No.
PF0421
Name
pDG (=pDP2)
Comment
Serotype: rep2/cap2 like pDP2rs, no RFP
Vector size
21.8 kbp
Item No.
PF0433.2
Name
pDP3.2
Comment
Serotype: rep2/cap3 like pDP3rs, no RFP
Vector size
21.9 kbp
Item No.
PF0434
Name
pDP4
Comment
Serotype: rep4/cap4 like pDP4rs, no RFP
Vector size
21.2 kbp
Item No.
PF0435
Name
pDP5
Comment
Serotype: rep2/cap5 like pDP5rs, no RFP
Vector size
21.8 kbp
Item No.
PF0436
Name
pDP6
Comment
Serotype: rep2/cap6 like pDP6rs, no RFP
Vector size
21.9 kbp
Item No.
PF0478
Name
pDP8.ape
Comment
Serotype: rep2/cap8
Vector size
22.0 kbp
Item No.
PF0439
Name
pDP9
Comment
Serotype: rep2/cap9
Vector size
21.8 kbp
Item No.
PF0422
Name
pDG (R484E/R585E)
Comment
Serotype: rep2/cap2 like pDG (PF0421), but without HSGAG binding motif
Vector size
19.2 kbp
Item No.
PF1451
Name
pAAV-ssGFP
Comment
vector plasmid with AAV-ITRs, CMV promotor, GFP, selection marker (Amp or Kan)
Vector size
8.0 kbp
Item No.
PF1500
Name
pAAV-scGFP
Comment
vector plasmid with AAV-ITRs, CMV promotor, GFP, selection marker (Amp or Kan)
Vector size
6.0 kbp
Item No.
MC3051
Name
MC.AAV-ssGFP
Comment
AAV-ITRs, CMV promotor, GFP, no bacterial backbone (e.g., no resistance genes)
Vector size
4.5 kbp
Item No.
MC3064
Name
MC.AAV-scGFP
Comment
AAV-ITRs, CMV promotor, GFP, no bacterial backbone (e.g., no resistance genes)
Vector size
2.5 kbp

The 2-Plasmid-System for AAV production offers a cost-effective solution, maximizing efficiency and minimizing expenses.

PlasmidFactory has obtained an exclusive, worldwide license for the production, distribution, and use of the Helper & Packaging plasmids of the pDG and pDP families from the German Cancer Research Centre (DKFZ).

DKFZ

AAV vector plasmids – keeping ITRs intact

PlasmidFactory’s proprietary ITRRESCUE® technology enables amplification of plasmid DNA containing sensi-tive inverted terminal repeats (ITR). While restriction digestion with SmaI is the standard quality control for ITR sequences, this method is not 100% accurate and does not allow quantification of the fraction of non-intact plasmids. PlasmidFactory’s self-developed Next Generation Sequencing (NGS) technology complements our fermentation process, allowing sequencing and quantification of ITR sequences while maintaining intact plasmids. GFP reporter gene cassettes with ITR2 flanked single strandet (ss) or self-complementary (sc) reporter genes are available In-Stock as minicircle or plasmid DNA.
(Fig. artistic representation)

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Gene Delivery Vehicles – AAVs for long-term gene expression

Adeno-associated viruses have great potential in the field of gene therapy. The use of minicircles improves the quality of viral vector preparations and increases production efficiency.

Scaling up: We offer DNA products of the highest purity in greater quantities than ever.

Read our large-scale story

When Minicircles were used for AAV production we got an increase of the genomic titer between 300-400% and an increase of the yield per cell.

Dr. Kathrin Teschner, Sartorius Xell on the positive impact of Minicircles on AAV production

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Useful background information

The most important terms relating to minicircle DNA and plasmids explained in detail. Useful background information for smooth communication.

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