Reporter constructs and pEPito plasmids

Plasmids to go – ready-to-use reporter gene plasmids and minicircles with GFP, luc, lacZ

We offer a variety of plasmids and minicircles with reporter genes and pEPito plasmids with S/MAR elements. These constructs are available from our In-Stock service for immediate delivery:

  • Guaranteed amount and reproducible quality with constant specifications of the plasmid DNA, including verified LPS-Endotoxin removal, and culture free from animal-derived media.
  • Precisely defined quality standards and homogeneous product, thanks to our proprietary CGE analysis technology for the quantification of different plasmid topologies.

GFP

GFP expression in skeletal mouse muscle (1)

luc

luc expression in mouse lung (2)

lacZ

lacZ expression in mouse tumour tissue (3)

Footnotes

[1] Trollet, Bigey and Scherman, Chemical and Genetic Pharmacology, Université René Descartes, Paris Ref. [1] and [2] in M. Schleef (ed.), DNA Pharmaceuticals, Wiley-VCH, 2005 [2] Rudolph, Maucksch and Aneja, Kinderklinik and Polyklinik, Dr. v. Haunersches Kinderspital, LMU München
[3] Walther and Stein, Max-Delbrück-Center for Molecular Medicine, Berlin

In-Stock:

Reporter plasmids & minicircles – ready to use!

All In-Stock products are pre-produced in Research grade quality and are therefore available at short notice. Minimum order quantity 5 mg (plasmid DNA) and 0.5 mg (minicircle DNA). Other quality grades on request.

Item No.NameSpecial featuresVector size
Item No.
PF0450
Name
pUC21
Special features
Control plasmid, Amp resistance
Vector size
3.2 kbp
Item No.
PF0451
Name
pUK21
Special features
Control plasmid, Amp resistance
Vector size
3.1 kbp
Item No.
PF0464
Name
pCMV
Special features
Control plasmid, CMV, Amp resistance
Vector size
3.5 kbp
Item No.
PF0461
Name
pCMV-luc
Special features
CMV, firefly luciferase, Amp resistance
Vector size
6.2 kbp
Item No.
PF0462
Name
pCMV-lacZ
Special features
CMV, β–galactosidase, Amp resistance
Vector size
7.2 kbp
Item No.
PF0463
Name
pCMV-GFP
Special features
CMV, GFP, Kan resistance
Vector size
3.5 kbp
Item No.
MC3048
Name
MC.CMV-luc
Special features
CMV, firefly luciferase, no bacterial backbone (e.g.,no resistance gene, no ori)
Vector size
3.6 kbp
Item No.
MC3290
Name
MC.CMV-lacZ
Special features
CMV, β–galactosidase, no bacterial backbone (e.g., no resistance gene, no ori)
Vector size
4.6 kbp
Item No.
MC3292
Name
MC.CMV-GFP
Special features
CMV, GFP, no bacterial backbone (e.g.,no resistance gene, no ori)
Vector size
1.8 kbp

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Insights

Precisely defined quality standards and homogeneous product, thanks to our proprietary CGE analysis technology for the quantification of different plasmid topologies.

McBox®

Reporter gene minicircles (GFP, luc, lacZ) are also available as McBox®.

Compare minicircle DNA with the corresponding plasmid in your experimental setting and optimize your transfection! The McBox® contains 100 µg each of certified minicircle and plasmid DNA, both of which include the reporter gene.

The McBox® is currently available carrying the following reporters: firefly luciferase gene (luc), green fluorescent protein (GFP), and the ß-galactosidase gene (lacZ).

The new McBox® provides scientists with the opportunity to directly compare minicircle DNA and the corresponding conventional plasmid in specific applications in their own labs.

McBox® GFP
(Item no. PFBox102)

MC.CMV-GFP
(minicircle, 100µg)

pCMV-GFP
(plasmid, 100µg)

WFI
(water for injection, 500µL)

McBox® luc
(Item no. PFBox101)

MC.CMV-luc
(minicircle, 100µg)

pCMV-luc
(plasmid, 100µg)

WFI
(water for injection, 500µL)

McBox® lacZ
(Item no. PFBox103)

MC.CMV-lacZ
(minicircle, 100µg)

pCMV-lacZ
(plasmid, 100µg)

WFI
(water for injection, 500µL)

Quote available on direct request

All In-Stock products are preproduced at Research grade and available with rapid delivery times. Other quality grades are available on request.

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pEPito plasmids with
S/MAR elements

S/MAR elements (scaffold/matrix attachment regions) are anchor sequences that link independently regulatable chromatin domains to the protein skeleton of the nucleus of eukaryotic cells.

In addition to supporting transcription and protecting against inactivation of (foreign) genetic information, a specific feature of these S/MAR elements has proven particularly important for the production of highly efficient plasmid DNA.

Plasmids that contain a S/MAR element inserted at a defined location, in addition to the therapeutically effective gene, are replicated episomally and passed on as independent units to the daughter cells of the eukaryotic target cell.

In other words, the plasmid is maintained as an episome for many generations, even in the absence of selection pressure, and will be stably expressed as an autonomous genetic unit without integrating.

The anchor sequence has been successfully reduced to a minimal motif of about 733 base pairs, without significantly affecting stable propagation to the daughter cells.

Corresponding plasmids and minicircles with S/MAR elements for stable gene expression (e.g. the pEPI and pEpito families) are marketed by PlasmidFactory via corresponding licensing agreements.

In-Stock:

pEPito plasmids

containing S/MAR elements

All In-Stock products are pre-produced in Research grade quality and are therefore available at short notice. Minimum order quantity 5 mg. Other quality grades on request.

Item No.NameSpecial featuresVector size
Item No.
PF1160
Name
pEPito-hCMV/EF1-EGFPLuc
Special features
R6K ori, hCMV enhancer, EF1 promotor, eGFP, firefly luciferase, S/MAR, Amp and Zeo resistance
Vector size
7.0 kbp
Item No.
PF1161
Name
pEPito-CMV-EGFPLuc
Special features
R6K ori, CMV-IEP, eGFP, firefly luciferase, S/MAR, Amp and Zeo resistance
Vector size
7.0 kbp
Item No.
PF1162
Name
pEPito-CMV-EGFP-IRES-BSD
Special features
R6K ori, CMV-IEP, eGFP, IRES, BSD, MARS, Amp and Zeo resistance
Vector size
5.8 kbp
Item No.
PF1163
Name
pEPito-hCMV/EF1-EGFP-IRES-BSD
Special features
R6K ori, hCMV enhancer, EF1 promotor, eGFP, IRES, BSD, S/MAR, Amp and Zeo resistance
Vector size
5.7 kbp
Item No.
PF1164
Name
pEPito-hCMV/EF1-EGFP
Special features
R6K ori, hCMV enhancer, EF1 promotor, eGFP, S/MAR, Amp and Neo resistance
Vector size
5.2 kbp
Item No.
PF1165
Name
pEPito-CMV-EGFP-IRES-BSD-∆S/MAR
Special features
R6K ori, CMV-IEP, eGFP, IRES, BSD, Amp and Zeo resistance
Vector size
3.9 kbp
Item No.
PF1166
Name
pEPito-hCMV/EF1-EGFP-IRES-BSD-∆S/MAR
Special features
R6K ori, hCMV enhancer, EF1 promotor, eGFP, IRES, BSD, Amp and Zeo resistance
Vector size
3.8 kbp

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Discover other In-Stock products: Helper & Packaging plasmids or vector plasmids & minicircles for AAV production.

The virtues of science are skepticism and independence of thought.

Walter Gilbert, Nobel Prize in Chemistry 1980
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