Customized MIDGE® DNA

June 06 | 2020

Minimized DNA in linear form with caps

An alternative concept for the production of minimal DNA vectors, which contain only the information necessary for the intended effect, is e.g. MIDGE® (minimal immunologically defined gene expression) DNA. The MIDGE structure is linear and tightly closed by single-stranded hairpin regions at both ends. This leads to the characteristic small size of these molecules, which – like the minicircles – have already been used in preclinical research as well as in clinical studies.

However, such linear molecules do not have the superhelicity characteristic of intact plasmids and PlasmidFactory‘s minicircles, so that minicircle DNA is, in our view, the preferred system when choosing a suitable DNA vector.

PlasmidFactory holds the worldwide trademark rights for the MIDGE technology.

MIDGE® features

• no bacterial backbone sequences (e.g. ori, antibiotic resistance marker)
• linear DNA fragment of minimal size
• highly purified
• reduced CpG content
• improved safety
• minimized side effects

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Useful background information

The most important terms relating to minicircle DNA and plasmids explained in detail. Useful background information for smooth communication.

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