Customized MIDGE® DNA

An alternative concept for the production of minimal DNA vectors, which contain only the information necessary for the intended effect, is e.g. MIDGE® (minimal immunologically defined gene expression) DNA. The MIDGE structure is linear and tightly closed by single-stranded hairpin regions at both ends. This leads to the characteristic small size of these molecules, which – like the minicircles – have already been used in preclinical research as well as in clinical studies.

However, such linear molecules do not have the superhelicity characteristic of intact plasmids and PlasmidFactory‘s minicircles, so that minicircle DNA is, in our view, the preferred system when choosing a suitable DNA vector.